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Biochemicals> | … then cultures were induced with 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) and cultured for a further 4.5 h. Recombinant Deg-AKR was purified from insoluble cell-lysates in the presence of 7 M urea by Ni affinity chromatography (Genscript Corporation, Piscataway, NJ … | Get A Quote |
The poultry red mite (PRM), Dermanyssus gallinae, is a hematophagous ectoparasite of birds with worldwide distribution that causes economic losses in the egg-production sector of the poultry industry. Traditional control methods, mainly based on acaricides, have been only partially successful, and new vaccine-based interventions are required for the control of PRM. Vaccination with insect Akirin (AKR) and its homolog in ticks, Subolesin (SUB), have shown protective efficacy for the control of ectoparasite infestations and pathogen infection/transmission. The aim of this study was the identification of the akr gene from D. gallinae (Deg-akr), the production of the recombinant Deg-AKR protein, and evaluation of i... More